This study represent a serious concern to the potential of zoonotic disease involving bacterial, parasitic dan viral infection by obtaining data of health status of monkey and human who are using monkey as performance monkey and to identify propablity of transmission pattern of zoonotic disease in their live and in their society. This research has conducted for two years and the samples have taken place in Jakarta, West Java (Bogor and Bandung), Central Java (Solo and Semarang) and East Java (Surabaya). The implementation of the research consist of three stages beginning with a survey to identify the location of the monkey, documentation of the source of zoonotic disease as a research biological samples targets, and detection for bacterial, viral, and parasitic infection. Genetic analysis is also conducted to generate effective management strategies and to assess future conservation and management programs throughout Indonesia. Our serologycal detection showed that out of 45 performance?s monkeys samples were positive to herpes simplex virus-1 (2.2%), hepatitis B virus (8.9%), dengue virus (4.4%), and simian T lymphotropic virus 1/2 (2.2%). Meanwhile, based on nucleotide detection by PCR and sequencing techniques indicated that 15.5% positive to Herpesvirus as Gammaherpesvirus and 2.2% positive to Rhabdovirus clustered as Novirhabdovirus. More than 50% of performance monkey?s samples taken from Jakarta were positive to endogen parasites. This finding proved the concept that humans and nonhuman primates have a close phylogenic relationship and have a high risk on diseases transfers. This research results are expected to provide a comprehensive data of the zoonotic disease appearing among the society of performance monkey and provide conservation of the long-tailed macaques.